Biofilm formation in bovine mastitis pathogens and the effect on them of antimicrobial drugs
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صندلی اداریAbstract
The ability of bacteria to produce a biofilm is considered an important virulent property in pathogenesis of mastitis. The purpose of studies is to investigate the ability to form biofilms, their density, to determine and compare the sensitivity to antibacterial drugs of planktonic and biofilm forms of the main bovine mastitis pathogens on dairy farms of the Western region of Ukraine.
Diagnosis of bovine mastitis, selection of milk samples and secretions of the mammary gland, microbiological studies were carried out in accordance with generally accepted methods. The performed studies have established that among pathogens, both acute and chronic forms of mastitis, the most productive film-forming ability had S. aureus strains, which on average 1.5 times more often formed the biofilm than Str. agalactiae and Str. dysgalactiae strains. It was revealed that S. aureus strains, isolated from cows under the subclinical form of mastitis and at carriage, 2.0 times (p <0.05) more often formed biofilms than in the clinical form of mastitis. The highest sensitivity of planktonic bacteria to pathogens of mastitis of streptococci and staphylococci was to ceftriaxone and doxycycline (100-80.9%). The least susceptible streptococci and staphylococci were to benzylpenicillin 32.3-45.4%, and the susceptibility of S. aureus strains was 19.0%.
When determining the influence of antibiotics on biofilm forms of bacteria found that cells in the biofilm are more resistant to antibacterial drugs. It was found that antibiotic enrofloxacin completely inactivated streptococci and staphylococci in biofilms. Also, antibiotics ceftriaxone and doxycycline were also effective on bacteria in biofilms. At the same time, under the action of antibiotics penicillins, aminoglycosides and macrolides, the amount of microbial cells that survived in a biofilm was about lg 5.3 CFU/cm2 of area. Consequently, studies have shown that it is necessary to seek effective methods and develop new drugs that would influence the bacteria in biofilms to effectively treat bovine mastitis.
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References
ASLANTAŞ, O.; DEMIR, C. (2016) Investigation of the antibiotic resistance and biofilm-forming ability of Staphylococcus aureus from subclinical bovine mastitis cases. Journal of dairy science, v. 99, n. 11, p. 8607-8613.
BENGTSSON, B.; UNNERSTAD, H. E.; EKMAN, T.; ARTURSSON, K.; NILSSON-ÖST, M.; WALLER, K. P. (2009) Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows. Veterinary microbiology, v. 136, n. 1-2, p. 142-149. DOI: 10.1016/j.vetmic.2008.10.024
FELIPE, V.; MORGANTE, C. A.; SOMALE, P. S.; VARRONI, F.; ZINGARETTI, M. L.; BACHETTI, R. A.; PORPORATTO, C. (2017) Evaluation of the biofilm forming ability and its associated genes in Staphylococcus species isolates from bovine mastitis in Argentinean dairy farms. Microbial pathogenesis, v. 104, p. 278-286. DOI: 10.1016/j.micpath.2017.01.047.
GOMES, F.; SAAVEDRA, M. J.; HENRIQUES, M. (2016) Bovine mastitis disease/pathogenicity: evidence of the potential role of microbial biofilms. Pathogens and Disease, v. 74, n. 3, p. 1-7. doi: 10.1093/femspd/ftw006
HORIUK, Y. V. (2018) Fagotherapy of cows mastitis as an alternative to antibiotics in the system of obtaining environmentally safe milk. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, v. 20, n. 88, p. 42-47. DOI: https://doi.org/10.32718/nvlvet8807
HORIUK, Y. V.; KUKHTYN, M. D.; STRAYSKYY, Y. S.; HAVRYLIANCHYK, R. Y.; HORIUK, V. V.; FOTINA, H. A. (2018) Comparison Of The Minimum Bactericidal Concentration Of Antibiotics On Planktonic And Biofilm Forms Of Staphylococcus Aureus: Mastitis Causative Agents. Res. J. Pharm., Biol. Chem. Sci, v. 9, n. 6, p. 616-622.
HORIUK, Y.; KUKHTYN, M.; PERKIY, Y.; HORIUK, V. (2018) Distribution of main pathogens of mastitis in cows on dairy farms in the western region of Ukraine. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, v. 20, n. 83, p. 115-119. doi: 10.15421/nvlvet8322
KEEFE, G. (2012) Update on control of Staphylococcus aureus and Streptococcus agalactiae for management of mastitis. Veterinary Clinics: Food Animal Practice, v. 28, n. 2, p. 203-216. DOI: https://doi.org/10.1016/j.cvfa.2012.03.010
KOVALENKO, V. L.; KOVALENKO, P. L.; PONOMARENKO, G. V.; KUKHTYN, M. D.; MIDYK, S. V.; HORIUK, Y. V.; GARKAVENKO, V. M. (2018) Changes in lipid composition of Escherichia coli and Staphylococcus areus cells under the influence of disinfectants Barez®, Biochlor® and Geocide®. Ukrainian Journal of Ecology, v. 8, n. 1, p. 547-550. DOI: http://dx.doi.org/10.15421/2018_248
KUKHTYN, M. D.; HORYUK, Y. V.; HORYUK, V. V.; YAROSHENKO, T. Y.; VICHKO, O. I.; POKOTYLO, O. S. (2017) Biotype characterization of Staphylococcus aureus isolated from milk and dairy products of private production in the western regions of Ukraine. Regulatory Mechanisms in Biosystems, v. 8, n. 3, p. 384-388. DOI: https://doi.org/10.15421/021759
KUKHTYN, M.; BERHILEVYCH, O.; KRAVCHENIUK, K.; SHYNKARUK, O.; HORYUK, Y.; SEMANIUK, N. (2017) Formation of biofilms on dairy equipment and the influence of disinfectants on them. Eastern-European Journal of Enterprise Technologies, v. 5, n. 11-89, p. 26-33. DOI: 10.15587/1729-4061.2017.110488
KUKHTYN, M.; BERHILEVYCH, O.; KRAVCHENIUK, K.; SHYNKARUK, O.; HORYUK, Y.; SEMANIUK, N. (2017) The influence of disinfectants on microbial biofilms of dairy equipment. EUREKA: Life Sciences, v. 5, p. 11-17. DOI: 10.21303/2504-5695.2017.00423
LAGO, A.; GODDEN, S. M. (2018) Use of Rapid Culture Systems to Guide Clinical Mastitis Treatment Decisions. Veterinary Clinics: Food Animal Practice, v. 34, n. 3, p. 389-412.
LIU, J.; YANG, L.; HOU, Y.; SOTEYOME, T.; ZENG, B.; SU, J.; LI, L.; LI, B.; CHEN, D.; LI, Y.; WU, A.; SHIRTLIFF, M. E.; HARRO, J. M.; XU, Z.; PETERS, B. M. (2018) Transcriptomics Study on Staphylococcus aureus Biofilm Under Low Concentration of Ampicillin. Frontiers in microbiology, v. 9, p. 2413. DOI: 10.3389/fmicb.2018.02413
NEOPANE P.; NEPAL H. P.; SHRESTHA R.; UEHARA O.; ABIKO Y. (2018) In vitro biofilm formation by Staphylococcus aureus isolated from wounds of hospital-admitted patients and their association with antimicrobial resistance. Int. J. Gen. Med, v. 11, p. 25–32. DOI: 10.2147/IJGM.S153268
ROYSTER, E.; WAGNER, S. (2015) Treatment of mastitis in cattle. Veterinary Clinics: Food Animal Practice, v. 31, n. 1, p. 17-46.
RUEGG, P. L. (2017) A 100-Year Review: Mastitis detection, management, and prevention. Journal of dairy science, v. 100, n. 12, p. 10381-10397.
RUEGG, P. L. (2018) Making Antibiotic Treatment Decisions for Clinical Mastitis. Veterinary Clinics: Food Animal Practice, v. 34, n. 3, p. 413-425.
RUEGG, P. L.; PETERSSON-WOLFE, C. S. (2018) Mastitis in dairy cows. Veterinary Clinics: Food Animal Practice, v. 34, n. 3, ix-x. DOI: https://doi.org/10.1016/j.cvfa.2018.08.001
SALISBURY, A. M.; WOO, K.; SARKAR, S.; SCHULTZ, G.; MALONE, M.; MAYER, D. O.; PERCIVAL, S. L. (2018) Tolerance of Biofilms to Antimicrobials and Significance to Antibiotic Resistance in Wounds. Surgical technology international, v. 11, n. 33, p. 59-66.
STEPANOVIC, S.; VUKOVIC, D.; DAKIC, I.; SAVIC, B.; SVABIC-VLAHOVIC, M. (2000). A modified microtiter-plate test for quantification of staphylococcal biofilm formation. Journal of microbiological methods, v. 40, n. 2, p. 175-179.