Adaptive changes in the ornithine cycle and amino acid synthesis in sheep liver with different meat productivity

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Tetiana Prylipko
Tetiana Koval
Volodymyr Kostash
Volodymyr Tkachuk
Alina Shuliar
Alona Shuliar
صندلی اداری

Abstract

The aim of the research was to study the ornithine cycle as the process of fixing ammonia and the formation of urea in the body of highly productive animals. In our experiments, we used a protein-deficient diet and urea as a nitrogen substitute for nitrogen-containing materials in the diet to reveal the mechanism of action of urea on animals, in particular on the biochemical processes of the ornithine cycle. There are some differences between Bukovinian sheep of the Askanian meat-wool breed and outbreds in terms of the ability to build muscle tissue. Our study reveals that the slaughter yield and the average daily gain consumption of Bukovinian-type meat of the Askanian meat-wool breed were higher in summer and in autumn, compare with purebred sheep. Sheep of the Bukovynian type of Askanian meat-wool breed have the intensity of enzymatic formation of urea in liver homogenates that is much higher in all experiments than in outbred sheep. A sharp drop in the activity of all stages of urea formation and glutamic acid synthesis in liver homogenates and significantly weakened urea formation was found in all experiments of the fourth series in comparison with the experiments in the third series. Increased muscle growth, high nitrogen deposition, and a much lower percentage of urinary excretion of ammonia and urea nitrogen, as well as higher activity of enzymes of the ornithine cycle and glutamic acid synthesis in the Bukovinian sheep type of Askanian meat-wool breed compared to outbreeds allow concluding that ammonia and urea in highly productive animals act less as finishing products of nitrogen metabolism than in low-productive animals.

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Integration System of Education, Science and Production
Author Biographies

Tetiana Prylipko, State Agrarian and Engіneering University in Podilya

Doctor Science in Agriculture, Professor, Head of the Department of Food Production Technologies and Food Standardization. She teaches disciplines taught: Modeling of technological processes of animal husbandry; Milk and dairy products technology. Author of more than 200 scientific works.

Tetiana Koval, State Agrarian and EngIneering University in Podilya

Ph.D. in Agriculture, Associate Professor at the Department Agrochemistry, chemical and general biological disciplinesю She teaches disciplines taught: Biochemistry of animals with basics of physical and colloid chemistryю Author of more than 110  scientific works.    

Volodymyr Kostash, State Agrarian and Engineering University in Podilya

Candidate of Agricultural Sciences (Ph.D.),  Assistant Professor, Department of Food Production Technologies and Food Standardization. He teaches disciplines taught: Animal carcass processing technology. Technological support and assessment of the suitability of premises for the production and processing of livestock products. Technological quality control and food safety. Food production technology. Primary milk processing technology. Commodity research, standardization. Organization of production processes. Author of more than  25 scientific works. 

Volodymyr Tkachuk, Zhytomyr National Agroecological University

Candidate of Agricultural Sciences (Ph.D.), Associate Professor at the Department Livestock Production Technologies of Animal Products. He teaches disciplines taught: technology of special meat cattle breeding, horse-making, technology of production of food products, technology of production of food products. Author of more than 121 scientific works. Web of Science ResearcherID A-5471-2018 

Alina Shuliar, Zhytomyr National Agroecological University

Ph.D. in Agriculture, Assistant at the Department of Livestock Production Technologies of Animal Products, Zhytomyr National Agroecological University. She teaches disciplines taught: technology of pig production, animal biology, animal biochemistry. Author of more than 71 scientific works.

Alona Shuliar, Zhytomyr National Agroecological University

Candidate of Agricultural Sciences (Ph.D.), Associate Professor at the Department Livestock Production Technologies of Animal Products, Zhytomyr National Agroecological University. She teaches disciplines taught: research and patenting methods, poultry production technology, modern research methods. Author of more than 124 scientific works. 

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